HPLC METHOD FOR SIMULTANEOUS DETERMINATION OF METFORMIN AND SITAGLIPTIN IN PHARMACEUTICAL DOSAGE FORMS AND ITS APPLICATIONS TO DISSOLUTION STUDY

Authors

  • Hemraj Sharma Department of Pharmaceutical Analysis and Quality Assurance, Raghavendra Institute of Pharmaceutical Education and Research [RIPER] Ananthapuramu, A.P, India.
  • Boya Madhuri Department of Pharmacy, Shree Medical and Technical College, Bharatpur, Nepal.
  • Yiragamreddy Padmanabha Reddy Department of Pharmaceutical Analysis and Quality Assurance, Raghavendra Institute of Pharmaceutical Education and Research [RIPER] Ananthapuramu, A.P, India.
  • Kondareddy Vinod Kumar Department of Pharmaceutical Analysis and Quality Assurance, Raghavendra Institute of Pharmaceutical Education and Research [RIPER] Ananthapuramu, A.P, India.
  • Nanda Kishor Bhatta Department of Pharmacy, Shree Medical and Technical College, Bharatpur, Nepal.
  • Bijaya Laxmi Giri Department of Pharmacy, Shree Medical and Technical College, Bharatpur, Nepal.

DOI:

https://doi.org/10.32553/ijmbs.v3i7.406

Keywords:

RP-HPLC, Metformin, Sitagliptin, Dissolution study

Abstract

A novel approach was used to develop and validate a rapid, specific, accurate and precise Reverse phase High performance liquid chromatographic (RP-HPLC) method for the simultaneous determination of Metformin and Sitagliptin in pharmaceutical dosage forms and its applications to dissolution study. The chromatographic separation was carried out on a C8 (250mm X 4.6 mm i.d., 5?m) column with a mobile phase of 40 Acetonitrile: 60 Phosphate Buffer (pH 6.8), using UV detector at 257 nm at 1ml min-1 flow rate. The retention time for Metformin was 2.11 minutes and 5.30 minutes for Sitagliptin. The Linearity for Metformin was found to be 10-80 µg ml-1 with R2 value of 0.9998 and for Sitagliptin 1-8 µg ml-1 with R2 value of 0.9976. Dissolution study of both for Metformin and Sitagliptin was carried, Percentage of drug release was established which was found to be 96.23% and 102.64% respectively, in the period of 50 minutes.

Keywords: RP-HPLC, Metformin, Sitagliptin, Dissolution study

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Published

2019-07-25

How to Cite

Sharma, H., Madhuri, B., Reddy, Y. P., Kumar, K. V., Bhatta, N. K., & Giri, B. L. (2019). HPLC METHOD FOR SIMULTANEOUS DETERMINATION OF METFORMIN AND SITAGLIPTIN IN PHARMACEUTICAL DOSAGE FORMS AND ITS APPLICATIONS TO DISSOLUTION STUDY. International Journal of Medical and Biomedical Studies, 3(7), 166-177. https://doi.org/10.32553/ijmbs.v3i7.406

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Section

Research Articles