PATTERN OF ANTI-U1-SN RNP ANTIBODY DISTRIBUTION USING LINE IMMUNOASSAY (LIA) PLATFORM IN PATIENTS’ ATTENDING A TERTIARY CARE HOSPITAL IN BIHAR
DOI:
https://doi.org/10.32553/ijmbs.v4i10.1515Abstract
Aims and Objective: To observe the age and sex wise distribution pattern of anti-U1snRNP antibody in patients attending AIIMS Patna using Line immunoassay platform (LIA), study the pattern of anti-U1sn RNP antibody positivity depending on intensity of bands observed and study the association of anti-U1snRNP antibody positivity with other antibodies comprising the ANA profile.
Material and Methods: A retrospective study on all samples received for ANA profile was done using line immunoassay (LIA) in Biochemistry central lab spanning the period from January 2019 to January 2020.Approximately 1200-1300 samples received for antibodies against nuclear antigens during this period were analysed for positivity of anti-U1snRNP antibody.Patient’s result data was categorised age and sex wise. The positivity of anti-U1snRNP will be graded as equivocal,(+),(++),(+++) depending on intensity of band with reference to cut off control. The anti-U1snRNP positivity will be recorded as single positivity or positivity along with other antibodies comprising the ANA profile.
Results: A total of 176 U1-SnRNP antibody positive patients were identified,1148 sera tested by LIA in which 588 samples were positive against 17 autoantigens (dsDNA,nucleosome,histone,SmD1,PCNA,P0,SS-A/Ro52,SS-A/Ro60,SS- B/La,CENP-B,Scl-70,U1-snRNP,AMA-M2,Jo-1,PM-Scl,Mi-2 and Ku).89 samples were identified for equivocal. So here we analysed only 255 samples,176 for U1-SnRNP positivity and 89 for equivocal. Sex distribution of 176 U1-SnRNP positive samples are showing in graph 1, 71% females and 29% males are positive against U1-SnRNP. we found that 77 females are positive for +1 and 18 for +2 while 30 for +3 grade which is significant.40 males +1,8 for +2 and 3 males showing+3 grade positivity. We found that 35.20% patients are between 25-40 years of age, 18.80% for pediatric age group,16.50% patients in15-24 yrs while 29.50% patients are above the 40 years of age.
Conclusion: Pattern of anti-U1-sn RNP antibody distribution using line immunoassay (LIA)as early markers for diagnosis of diseases.
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