EFFICACY OF NOVEL ROOT CANAL IRRTIGANT-MTAD AGAINST ROOT BIOMODIFIERS ON PERIODONTALLY INVOVLED TEETH IN PERIODONTAL REGENRATION- AN IN VITRO SCANNING ELECTRON MICROSCOPY STUDY.

Authors

  • Dasarapu Srujan Department of Periodontics and Implantology, Divya Jyoti college of Dental Sciences and research, Modinagar, Uttar Pradesh, India.
  • Gaurav Malhotra Department of Periodontics and Implantology, Divya Jyoti college of Dental Sciences and research, Modinagar, Uttar Pradesh, India.
  • Pradeep Shukla Department of Periodontics and Implantology, Divya Jyoti college of Dental Sciences and research, Modinagar, Uttar Pradesh, India.
  • Prerna Kataria Department of Periodontics and Implantology, Divya Jyoti college of Dental Sciences and research, Modinagar, Uttar Pradesh, India.
  • Mona Dagar Department of Periodontics and Implantology, Divya Jyoti college of Dental Sciences and research, Modinagar, Uttar Pradesh, India.

DOI:

https://doi.org/10.32553/ijmbs.v5i1.1619

Keywords:

Citric acid, ethylenediaminetetraacetic acid, MTAD, root biomodifier, scanning electron microscope study, tetracycline hydrochloride

Abstract

Background: Smear layer removal and collagen fiber exposure may improve regeneration which can be accomplished by use of root biomodifiers. These enhance the degree of connective tissue attachment to denuded roots. The objective of this in vitro study was to evaluate and compare novel root canal irrigant and other root biomodifiers for smear layer removal on periodontally involved human teeth.

Materials and Methods: Forty human teeth were collected and stored in saline. After scaling and root planing, two samples were obtained from each tooth. A total of 100 dentin blocks were randomly divided into four groups: Mixture of tetracycline, acid and detergent (MTAD), tetracycline hydrochloride (TTC HCl), citric acid (CA), and ethylenediaminetetraacetic acid (EDTA). The agents were applied for 3 min by active burnishing. Immediately following treatment, the specimens were rinsed, dehydrated, fixed and prepared for scanning electron microscope and were examined at × 3500 magnification. Sampaio’s index was evaluated by the previously trained blind examiner using photomicrographs.  Groups  were  compared  using  analysis  of  variance  followed  by  Tukey’s  post?hoc  test.

Results: Mixture of tetracycline, acid, and detergent is most efficacious in removing the smear layer and showed statistically significant dentinal tubules opening, followed by EDTA, TTC HCl, and CA.

Conclusion: Mixture of tetracycline, acid and detergent and conventional root biomodifiers used in the study alters the dentin surface by smear layer removal and exposure of dentinal tubules. Hence, MTAD as a root biomodifier may have a significant role in periodontal regeneration.

Key words: Citric acid, ethylenediaminetetraacetic acid, MTAD, root biomodifier, scanning electron microscope study, tetracycline hydrochloride

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Published

2021-01-06

How to Cite

Srujan, D. ., Malhotra, G. ., Shukla, P. ., Kataria, P. ., & Dagar, M. . (2021). EFFICACY OF NOVEL ROOT CANAL IRRTIGANT-MTAD AGAINST ROOT BIOMODIFIERS ON PERIODONTALLY INVOVLED TEETH IN PERIODONTAL REGENRATION- AN IN VITRO SCANNING ELECTRON MICROSCOPY STUDY. International Journal of Medical and Biomedical Studies, 5(1). https://doi.org/10.32553/ijmbs.v5i1.1619

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