Estimation of therapeutic levels of Imatinib and its correlation with clinical response has become important to monitor response of this drug in chronic myeloid leukemia (CML) patients. Various attempts have been made to develop quantitative protocols for imatinib in human serum using different analytical tools. A simple, sensitive and specific quantitative method was developed in the present study for monitoring of imatinib levels in human serum by applying high performance liquid chromatography (HPLC) and QQQ tandem mass spectrometry. An efficient simple sample extraction method was developed using solid phase extraction (SPE) method for extraction of imatinib in human serum with a recovery of around 80-85%. The developed method was successfully tested on 30 human serum samples who have been receiving imatinib for CML.
Keywords: Imatinib, LCMS, MRM, methanol, serum